ACTH Antibody (CLIP/7197R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-24042

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Kappa Clone # CLIP/7197R

Format

Azide and BSA Free
View all ACTH Primary Antibodies »

Product Specifications

Immunogen

N-terminal fragment of human ACTH conjugated to KLH

Reactivity Notes

Expected to show a broad species reactivity.

Localization

Cytoplasm. Secreted.

Marker

Pituitary Marker

Specificity

This monoclonal antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for ACTH Antibody (CLIP/7197R) - Azide and BSA Free

Immunohistochemistry-Paraffin: ACTH Antibody (CLIP/7197R) - Azide and BSA Free [NBP3-24042] -

Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH antibody (CLIP/7197R) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for ACTH Antibody (CLIP/7197R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ACTH

ACTH occurs in cells of the anterior pituitary and in neurons in brain. It regulates the corticosteroid production in the adrenal cortex. Beta endorphin and Met enkephalin are endogenous opiates. MSH (melanocyte stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.

Long Name

Adrenocorticotropic Hormone

Alternate Names

Adrenocorticotropin, Corticotropin

Gene Symbol

POMC

Additional ACTH Products

Product Documents for ACTH Antibody (CLIP/7197R) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ACTH Antibody (CLIP/7197R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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