Adenine Nucleotide Translocase 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92642

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Adenine Nucleotide Translocase 1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 101-200 of human Adenine Nucleotide Translocase 1 (NP_001142.2). VGKGAAQREFHGLGD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Adenine Nucleotide Translocase 1 Antibody - BSA Free

Western Blot: Adenine Nucleotide Translocase 1 AntibodyAzide and BSA Free [NBP2-92642]

Western Blot: Adenine Nucleotide Translocase 1 AntibodyAzide and BSA Free [NBP2-92642]

Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP2-92642] - Analysis of extracts of various cell lines, using SLC25A4/ANT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Adenine Nucleotide Translocase 1 AntibodyAzide and BSA Free [NBP2-92642]

Western Blot: Adenine Nucleotide Translocase 1 AntibodyAzide and BSA Free [NBP2-92642]

Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP2-92642] - Analysis of extracts of MCF7 cells, using SLC25A4/ANT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Knockout Validated: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92642]

Western Blot: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92642]

Western Blot: Adenine Nucleotide Translocase 1 Antibody [NBP2-92642] - Analysis of extracts of 293T cells, using SLC25A4/ANT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92642] -

Immunocytochemistry/ Immunofluorescence: Adenine Nucleotide Translocase 1 Antibody - Azide and BSA Free [NBP2-92642] - Immunofluorescence analysis of NIH/3T3 cells using Adenine Nucleotide Translocase 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Adenine Nucleotide Translocase 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Reviewed Applications

Read 1 review rated 1 using NBP2-92642 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Adenine Nucleotide Translocase 1

Adenine Nucleotide Translocase 1 (ANT1) catalyzes the exchange of ADP and ATP across the mitochondrial inner membrane. It is is a key component of the mitochondrial permeability transition pore (PT pore) complex and is also one of the most abundant proteins in the mitochondrion making up approximately 10% of all mitochondrial proteins. Originally described as an ADP/ATP exchange factor, ANT1 has also been shown to be required for bax mediated apoptosis. This is effected by binding of bax to residues 105-156 of ANT1, presumably resulting in maintaining the PT pore in an open conformation. Interaction with bax is not required for ANT1 pro-apoptotic activity as overexpression of the ANT1 protein also leads to the induction of apoptosis.

Alternate Names

AAC1, Adenine nucleotide translocator 1, ADP, ADP/ATP translocase 1, ANT 1, ANT1adenine nucleotide translocator 1 (skeletal muscle), ATP carrier protein 1, ATP carrier protein, heart/skeletal muscle, ATP carrier protein, heart/skeletal muscle isoform T1, heart/skeletal muscle ATP/ADP translocator, PEO2, PEO3, solute carrier family 25 (mitochondrial carrier; adenine nucleotidetranslocator), member 4, Solute carrier family 25 member 4, T1ANT

Gene Symbol

SLC25A4

Additional Adenine Nucleotide Translocase 1 Products

Product Documents for Adenine Nucleotide Translocase 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Adenine Nucleotide Translocase 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Adenine Nucleotide Translocase 1 Antibody - BSA Free

Customer Reviews for Adenine Nucleotide Translocase 1 Antibody - BSA Free (1)

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  • Not working with Jess (Simple Western)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: pulmonary artery endothelial cells
    Species: Human
    Verified Customer | Posted 08/28/2024
    Unspecific bands for ANT1
    PAEC exposed to normoxia (21% O2) and hypoxia (1% O2).
    Adenine Nucleotide Translocase 1 Antibody - BSA Free NBP2-92642
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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