AKAP9 Antibody (17G10)

Novus Biologicals | Catalog # NB100-74351

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 17G10
View all AKAP9 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues Q(31) F R Q R K A Q S D G Q S P S(45) of human AKAP450.

Specificity

AKAP450 (17G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for AKAP9 Antibody (17G10)

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351]

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351]

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351] - Analysis of AKAP450 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: AKAP9 Antibody (17G10) [NB100-74351]

Immunohistochemistry-Paraffin: AKAP9 Antibody (17G10) [NB100-74351]

Immunohistochemistry-Paraffin: AKAP9 Antibody (17G10) [NB100-74351] - Normal biopsies of deparaffinized Human pancreas tissue at a dilution of 1:20.
Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351]

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351]

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351] - Analysis of AKAP450 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351]

Immunocytochemistry/ Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351]

Immunocytochemistry/Immunofluorescence: AKAP9 Antibody (17G10) [NB100-74351] - Analysis of AKAP450 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP450 monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. AKAP450 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Applications for AKAP9 Antibody (17G10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

4 ug/mL

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:10 - 1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

2 ug/mL

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AKAP9

AKAP450 (A-Kinase anchoring protein 450) is a coiled-coil, scaffolding protein. It is a member of a group of AKAP's, derived from a spliced gene located on human choromosome 7. AKAP450 regulates kinases which participate in centrosomal structure and regulation. AKAP450 also functions as an MR-1 binding protein with potential action in cytoskeletal attachment of the NMDA receptor. It has also been found to be specifically concentrated at the skeletal muscle and neuromuscular junction.

Alternate Names

A kinase (PRKA) anchor protein (yotiao) 9, AKAP 350, AKAP 450, AKAP350A-kinase anchor protein 450 kDa, AKAP450A-kinase anchor protein 350 kDa, AKAP-9, Centrosome- and Golgi-localized PKN-associated protein, CG-NAPAKAP 120-like protein, hgAKAP 350, HYPERION, KIAA0803A-kinase anchor protein 9, MU-RMS-40.16A, PRKA9AKAP9-BRAF fusion protein, Protein hyperion, protein kinase A anchoring protein 9, Protein kinase A-anchoring protein 9, Protein yotiao, YOTIAOkinase N-associated protein

Entrez Gene IDs

10142 (Human)

Gene Symbol

AKAP9

Additional AKAP9 Products

Product Documents for AKAP9 Antibody (17G10)

Certificate of Analysis

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Product Specific Notices for AKAP9 Antibody (17G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for AKAP9 Antibody (17G10)

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