Aldolase C Antibody (4A9) - BSA Free

Novus Biologicals | Catalog # NBP2-25145

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4A9

Format

BSA Free
View all Aldolase C Primary Antibodies »

Product Specifications

Immunogen

The N-terminal 20 amino acids of human Aldolase C (MPHSYPALSAEQKKELSDIA). [UniProt# P09972]

Localization

Gray matter astrocytes and cells of the pia mater

Marker

pan-Astrocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aldolase C Antibody (4A9) - BSA Free

Western Blot: Aldolase C Antibody (4A9) [NBP2-25145]

Western Blot: Aldolase C Antibody (4A9) [NBP2-25145]

Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Human breast cancer MCF7 cells transfected with Aldolase C guide RNA. Western blot image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145]

Immunocytochemistry/ Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145]

Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - A. Immunofluorescence of rat cerebellum section stained with mouse mAb to aldolase C, NBP2-25145, dilution 1:1000, in green, and costained with rabbit pAb to GFAP, dilution 1:5000 in red. In both A and B blue is Hoechst staining of nuclear DNA. Aldolase C antibody selectively labels the perikarya and dendrites of Purkinje cells, while GFAP antibody stains processes of Bergman glia and astrocytic cells. B. Immunofluorescence of E20 rat cortical culture stained with NBP2-25145, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:10,000 in red. The aldolase C antibody labels cytosolic protein expressed in glial cells, while MAP2 antibody stains dendrites and perikarya of mature neurons.
Western Blot: Aldolase C Antibody (4A9) [NBP2-25145]

Western Blot: Aldolase C Antibody (4A9) [NBP2-25145]

Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Analysis of tissue and lysates using mouse mAb to aldolase C, NBP2-25145, dilution 1:2000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, and [5] pig hippocampus. The single band at about 40 kDa corresponds to the aldolase C protein.
Immunocytochemistry/ Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145]

Immunocytochemistry/ Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145]

Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - View of mixed neuron/glial cultures stained with NBP2-25145 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25145 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.

Applications for Aldolase C Antibody (4A9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:2000
Application Notes
This Aldolase C (1A1) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~40 kDa.

Reviewed Applications

Read 1 review rated 5 using NBP2-25145 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aldolase C

Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene. Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors. In the adult, Aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues. Aldolase C shares 81% amino acid identity with Aldolase A and 70% identity with Aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that Aldolase A and C are expressed in complementary cell types: Aldolase A mRNA is found in neurons; Aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cella. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific.

Alternate Names

ALDC, aldolase 3, aldolase C, fructose-bisphosphate, Brain-type aldolase, EC 4.1.2.13, fructoaldolase C, fructose-1,6-biphosphate triosephosphate lyase, fructose-bisphosphate aldolase C

Entrez Gene IDs

230 (Human)

Gene Symbol

ALDOC

OMIM

103870 (Human)

UniProt

P09972 (Human)

Additional Aldolase C Products

Product Documents for Aldolase C Antibody (4A9) - BSA Free

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Product Specific Notices for Aldolase C Antibody (4A9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aldolase C Antibody (4A9) - BSA Free

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  • Aldolase C Antibody (4A9)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: breast cancer MCF7 cells
    Species: Human
    Verified Customer | Posted 10/22/2019
    MCF-7 transfected with Aldolase c guide RNA
    Aldolase C Antibody (4A9) - BSA Free NBP2-25145

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