AMPK alpha 2 Antibody (1G8) - Azide and BSA Free

Novus Biologicals | Catalog # H00005563-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G8

Format

Azide and BSA Free
View all AMPK alpha 2 Primary Antibodies »

Product Specifications

Immunogen

PRKAA2 (NP_006243, 453 a.a. ~ 552 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR

Specificity

PRKAA2 - protein kinase, AMP-activated, alpha 2 catalytic subunit

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AMPK alpha 2 Antibody (1G8) - Azide and BSA Free

Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of PRKAA2 expression in transfected 293T cell line by PRKAA2 monoclonal antibody (M02), clone 1G8.Lane 1: PRKAA2 transfected lysate(62.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Immunocytochemistry/ Immunofluorescence: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Immunocytochemistry/Immunofluorescence: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of monoclonal antibody to PRKAA2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of PRKAA2 over-expressed 293 cell line, cotransfected with PRKAA2 Validated Chimera RNAi ( Cat # H00005563-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKAA2 monoclonal antibody (M02) clone 1G8 (Cat # H00005563-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Proximity Ligation Assay: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Proximity Ligation Assay: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

Proximity Ligation Assay: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of protein-protein interactions between STK11 and PRKAA2. HeLa cells were stained with anti-STK11 rabbit purified polyclonal 1:1200 and anti-PRKAA2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

ELISA: AMPK alpha 2 Antibody (1G8) [H00005563-M02]

ELISA: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Detection limit for recombinant GST tagged PRKAA2 is approximately 0.03ng/ml as a capture antibody.

Applications for AMPK alpha 2 Antibody (1G8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AMPK alpha 2

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Long Name

AMP-activated Protein Kinase alpha

Alternate Names

PRKAA2

Entrez Gene IDs

5563 (Human)

Gene Symbol

PRKAA2

OMIM

600497 (Human)

UniProt

NP_006243

Additional AMPK alpha 2 Products

Product Documents for AMPK alpha 2 Antibody (1G8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for AMPK alpha 2 Antibody (1G8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Intracellular Kinases
Obesity

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Protocols

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FAQs

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