Anti- Cetuximab (Anti-Idiotype) Antibody
R&D Systems | Catalog # MAB9626
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Multi-Species
Cited:
Human
Applications
Western Blot, ELISA, Flow Cytometry, Simple Western
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2259C
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Product Specifications
Immunogen
Full length Cetuximab
Specificity
Detects Cetuximab in Western blots and direct ELISAs. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Anti- Cetuximab (Anti-Idiotype) Antibody
Detection of Cetuximab by Western Blot.
Western blot shows Cetuximab, Rituximab, Brentuximab, and Gemtuzumab biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cetuximab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of anti-EGFR/ErbB1 (Cetuximab) on A431 Human Cell Line by Flow Cytometry.
A431 human epithelial carcinoma cell line was stained with anti-EGFR/ErbB1 (Cetuximab) (Catalog # MAB9577), then anti-Cetuximab (Anti-Idiotype) (Catalog # MAB9626, filled histogram) or Normal Rabbit IgG Control (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).
Specificity of Anti-Cetuximab (Anti-Idiotype) Antibody in Direct ELISA
A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Cetuximab (Anti-Idiotype) Antibody (Catalog # MAB9626, 333 ng/mL) detected Cetuximab. No cross-reactivity with Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab, Teprotumumab, and Aducanumab was observed.
Detection of Cetuximab by Simple WesternTM.
Simple Western lane view shows Cetuximab and Rituximab biosimilars, loaded at 0.2 mg/mL. A specific band was detected for Cetuximab at approximately 174 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Anti- Cetuximab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9626). This experiment was conducted under non-reducing conditions and using the 66-440 kDa separation system.Applications for Anti- Cetuximab (Anti-Idiotype) Antibody
Application
Recommended Usage
ELISA
Detects Cetuximab in direct ELISAs.
Flow Cytometry
0.25 µg/106 cells
Sample: A431 Human cell line stained with Anti-EGFR/ErbB1 (Cetuximab), Catalog # MAB9577
Sample: A431 Human cell line stained with Anti-EGFR/ErbB1 (Cetuximab), Catalog # MAB9577
Simple Western
20 µg/mL
Sample: Cetuximab under non-reducing conditions only
Sample: Cetuximab under non-reducing conditions only
Western Blot
0.5 µg/mL
Sample: Cetuximab under non-reducing conditions only
Sample: Cetuximab under non-reducing conditions only
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cetuximab
Additional Cetuximab Products
Product Documents for Anti- Cetuximab (Anti-Idiotype) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Anti- Cetuximab (Anti-Idiotype) Antibody
For research use only
Citations for Anti- Cetuximab (Anti-Idiotype) Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Anti- Cetuximab (Anti-Idiotype) Antibody
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Q: What is an anti-idiotype antibody?
A: An anti-idiotype antibody is specific for the unique variable (antigen binding) region of another antibody. We have anti-idiotype antibodies to commecially available therapeutic antibodies or biosimilars.
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