Q: Are any of your Aquaporin-1 antibodies suitable for live cell staining? I am looking at doing flow cytometry on live cells and further isolation of the positive cells to purify the population.

A: A list of our Aquaporin-1 antibodies suitable for use in flow cytometry can be found using this link. Of note, NB600-741 is known to bind to an extracellular domain, so should be suitable for your use.

Aquaporin 1/AQP1 Antibody (S03-7G5)

Novus Biologicals | Catalog # NBP3-19572

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-7G5 expressed in HEK293

View all Aquaporin 1/AQP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Aquaporin 1/AQP1 (Uniprot # P29972)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aquaporin 1/AQP1 Antibody (S03-7G5)

Western Blot: Aquaporin 1/AQP1 Antibody (S03-7G5) [NBP3-19572]

Western Blot: Aquaporin 1/AQP1 Antibody (S03-7G5) [NBP3-19572] - Western blot detection of Aquaporin 1/AQP1 in C6 cell lysates using NBP3-19572 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 29kDa.

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (S03-7G5) [NBP3-19572]

Immunohistochemistry-Paraffin: Aquaporin 1/AQP1 Antibody (S03-7G5) [NBP3-19572] - Immunohistochemistry of Aquaporin 1/AQP1 in paraffin-embedded Human breast cancer tissue using NBP3-19572 at dilution 1/100

Applications for Aquaporin 1/AQP1 Antibody (S03-7G5)

Application

Recommended Usage

Immunohistochemistry

1/50-1/200

Western Blot

1/1000-1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aquaporin 1/AQP1

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Thisgene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayerspanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant inerythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involvingimbalance in ocular fluid movement. (provided by RefSeq)

Alternate Names

AQP1, CHIP28, CO

Gene Symbol

AQP1

Additional Aquaporin 1/AQP1 Products

Product Documents for Aquaporin 1/AQP1 Antibody (S03-7G5)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aquaporin 1/AQP1 Antibody (S03-7G5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

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