Aquaporin-9 Antibody

Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865]

Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human stomach tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865]

Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human testis tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865]

Immunohistochemistry-Paraffin: Aquaporin-9 Antibody [NBP1-30865] - IHC on paraffin sections of human brain tissue. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturer's instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer). Sections were counterstained with Harris Hematoxylin.

Western Blot: Aquaporin-9 Antibody [NBP1-30865] -

AQP9 and ZAP70 up-regulation inhibits cell viability of laryngeal cancer. A, B RT-qPCR for AQP9 and ZAP70 mRNA expression in TU212 and LCC cells transfected with siRNAs against AQP9 and ZAP70 or their overexpression plasmids. C-E Western blot for AQP9 and ZAP70 expression in TU212 and LCC cells transfected with siRNAs against AQP9 and ZAP70 or their overexpression plasmids. F, G CCK-8 for cell viability of TU212 and LCC cells transfected with si-AQP9 or AQP9 overexpression plasmid. H, I CCK-8 for cell viability of TU212 and LCC cells transfected with si-ZAP70 or ZAP70 overexpression plasmid. *P < 0.05; **P < 0.01; ***p < 0.001; ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35477402), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Aquaporin-9 Antibody [NBP1-30865] -

Validation of AQP9 and ZAP70 expression in laryngeal cancer. A, B RT-qPCR for expression of AQP9 and ZAP70 in each laryngeal cancer sample. C, D RT-qPCR for expression of AQP9 and ZAP70 in laryngeal cancer and normal tissues. E, F ROC curves of AQP9 and ZAP70 expression for assessing the diagnostic value of their expression. G-J Immunohistochemistry analysis of expression of AQP9 and ZAP70 in laryngeal cancer and normal tissues. Scale bar, 50 μm. K, L RT-qPCR for expression of AQP9 and ZAP70 in HaCaT, TU212 and LCC cells. M-P Immunohistochemistry analysis of expression of AQP9 and ZAP70 in HaCaT, TU212 and LCC cells. Scale bar, 50 μm. *P < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35477402), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Aquaporin-9 Antibody [NBP1-30865] -

Validation of AQP9 and ZAP70 expression in laryngeal cancer. A, B RT-qPCR for expression of AQP9 and ZAP70 in each laryngeal cancer sample. C, D RT-qPCR for expression of AQP9 and ZAP70 in laryngeal cancer and normal tissues. E, F ROC curves of AQP9 and ZAP70 expression for assessing the diagnostic value of their expression. G-J Immunohistochemistry analysis of expression of AQP9 and ZAP70 in laryngeal cancer and normal tissues. Scale bar, 50 μm. K, L RT-qPCR for expression of AQP9 and ZAP70 in HaCaT, TU212 and LCC cells. M-P Immunohistochemistry analysis of expression of AQP9 and ZAP70 in HaCaT, TU212 and LCC cells. Scale bar, 50 μm. *P < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35477402), licensed under a CC-BY license. Not internally tested by Novus Biologicals.