ARFGAP1 Antibody (1C4E2) - BSA Free

Novus Biologicals | Catalog # NBP2-61670

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1C4E2

Format

BSA Free
View all ARFGAP1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human ARFGAP1 (AA: 270-414) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

44.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARFGAP1 Antibody (1C4E2) - BSA Free

Western Blot: ARFGAP1 Antibody (1C4E2)BSA Free [NBP2-61670]

Western Blot: ARFGAP1 Antibody (1C4E2)BSA Free [NBP2-61670]

Western Blot: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis using ARFGAP1 mAb against HEK293 (1) and ARFGAP1 (AA: 270-414)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: ARFGAP1 Antibody (1C4E2) - BSA Free [NBP2-61670]

Flow Cytometry: ARFGAP1 Antibody (1C4E2) - BSA Free [NBP2-61670]

Flow Cytometry: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis of HepG2 cells using ARFGAP1 mouse mAb (green) and negative control (red).
Western Blot: ARFGAP1 Antibody (1C4E2)BSA Free [NBP2-61670]

Western Blot: ARFGAP1 Antibody (1C4E2)BSA Free [NBP2-61670]

Western Blot: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis using ARFGAP1 mAb against human ARFGAP1 (AA: 270-414) recombinant protein. (Expected MW is 41.5 kDa)
Western Blot: ARFGAP1 Antibody (1C4E2)BSA Free [NBP2-61670]

Western Blot: ARFGAP1 Antibody (1C4E2)BSA Free [NBP2-61670]

Western Blot: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Analysis using ARFGAP1 mouse mAb against MOLT4 (1), C2C12 (2), HepG2 (3), MCF-7 (4), and Lncap (5) cell lysate.
ELISA: ARFGAP1 Antibody (1C4E2) - BSA Free [NBP2-61670]

ELISA: ARFGAP1 Antibody (1C4E2) - BSA Free [NBP2-61670]

ELISA: ARFGAP1 Antibody (1C4E2) [NBP2-61670] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for ARFGAP1 Antibody (1C4E2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Western Blot

1:100-1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARFGAP1

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.

Long Name

ADP-ribosylation factor GTPase-activating protein 1

Alternate Names

ARF GAP 1, ARF1 GAP, ARF1GAP

Gene Symbol

ARFGAP1

Additional ARFGAP1 Products

Product Documents for ARFGAP1 Antibody (1C4E2) - BSA Free

Certificate of Analysis

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Product Specific Notices for ARFGAP1 Antibody (1C4E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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