ARFGEF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88840

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ARFGEF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ARFGEF1 Antibody - BSA Free

Western Blot: ARFGEF1 Antibody [NBP1-88840]

Western Blot: ARFGEF1 Antibody [NBP1-88840]

Western Blot: ARFGEF1 Antibody [NBP1-88840] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: ARFGEF1 Antibody [NBP1-88840]

Immunocytochemistry/ Immunofluorescence: ARFGEF1 Antibody [NBP1-88840]

Immunocytochemistry/Immunofluorescence: ARFGEF1 Antibody [NBP1-88840] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840]

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840]

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] - Staining of human cerebral cortex shows cytoplasmic immunoreactivity in neuronal cell bodies.
ARFGEF1 Antibody

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] -

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] -Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
ARFGEF1 Antibody

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] -

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
ARFGEF1 Antibody

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] -

Immunohistochemistry-Paraffin: ARFGEF1 Antibody [NBP1-88840] -Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for ARFGEF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ARFGEF1

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP. It contains a Sec7 domain, which may be responsible for the guanine-nucleotide exchange activity and also the brefeldin A inhibition.

Alternate Names

ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldinA-inhibited), ARFGEP1P200, BIG1p200, Brefeldin A-inhibited GEP 1, brefeldin A-inhibited guanine nucleotide-exchange protein 1, D730028O18Rik, DKFZP434L057, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1

Gene Symbol

ARFGEF1

Additional ARFGEF1 Products

Product Documents for ARFGEF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ARFGEF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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