ARPC2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92039

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ARPC2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ARPC2 (NP_690601.1). MILLEVNNRIIEETLALKFENAAAGNKPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKELQAHGADELLKRVYGSFLVNPESGYNVSLLYDLENLPASKDSIVHQAGMLKRNCFASVFEKYFQFQEEGKEGENRAVIHYRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEFKEGRRASHTAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNASARDNTINLIHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPDAEKKEMKTITGKTFSSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ARPC2 Antibody - Azide and BSA Free

Western Blot: ARPC2 AntibodyAzide and BSA Free [NBP2-92039]

Western Blot: ARPC2 AntibodyAzide and BSA Free [NBP2-92039]

Western Blot: ARPC2 Antibody [NBP2-92039] - Analysis of extracts of various cell lines, using ARPC2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.
Immunohistochemistry-Paraffin: ARPC2 Antibody - Azide and BSA Free [NBP2-92039]

Immunohistochemistry-Paraffin: ARPC2 Antibody - Azide and BSA Free [NBP2-92039]

Immunohistochemistry-Paraffin: ARPC2 Antibody [NBP2-92039] - Immunohistochemistry of paraffin-embedded rat spleen using ARPC2 Rabbit pAb (NBP2-92039) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for ARPC2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ARPC2

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.

Alternate Names

actin related protein 2/3 complex, subunit 2 (34 kD), actin related protein 2/3 complex, subunit 2, 34kDa, ARC34actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subunit, ARP2/3 protein complex subunit 34, p34-Arc, PNAS-139

Gene Symbol

ARPC2

Additional ARPC2 Products

Product Documents for ARPC2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ARPC2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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