ASC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89656

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ASC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ILQRDSNKSQKLLKKLMSGVENSGKVDISTKDLLPHQELRIKSGLEKAIKHKDKLLEFDRTSIRRTQVIDDESDYFASDSNQWLSKLERETLQKREEELRELRHASRLSKKVTIDFAGRKILEEENSLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASC1 Antibody - BSA Free

ASC1 Antibody - BSA Free Immunohistochemistry-Paraffin: ASC1 Antibody - BSA Free [NBP1-89656]

Immunohistochemistry-Paraffin: ASC1 Antibody - BSA Free [NBP1-89656]

Staining of human colon shows cytoplasmic positivity in glandular cells.
ASC1 Antibody - BSA Free Western Blot: ASC1 Antibody - BSA Free [NBP1-89656]

Western Blot: ASC1 Antibody - BSA Free [NBP1-89656]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
ASC1 Antibody - BSA Free

Western Blot: ASC1 Antibody - BSA Free [NBP1-89656] -

Liraglutide inhibits glucolipotoxicity-induced NF kappa B pro-inflammatory responses. (a) Western blots showed that 0.1 μM Liraglutide inhibits glucolipotoxicity-induced NF kappa B p65 subunit phosphorylation at Ser536 and prevents I kappa B alpha degradation at 24 h. (b) Double fluorescence staining revealed that Liraglutide inhibits the entry of the NF kappa B p65 subunit into the nucleus under glucolipotoxicity. Scale bar: 20 μm. (c) qPCR results demonstrated that under glucolipotoxicity, liraglutide significantly reduces the mRNA expression levels of the IL-1 beta, TNF alpha, and MCP-1 pro-inflammatory cytokines, and simultaneously restores the IL-4, IL-10, and IGF-1 anti-inflammatory cytokines. (d) Western blot analysis of NLRP3, ASC, and cleaved-caspase-1 confirmed that Liraglutide suppresses glucolipotoxicity-induced inflammasome activation. Arrows indicate nuclear p65 immunolabeling. All values are presented as the mean +/- SEM. Significant difference was determined using multiple comparisons of Dunnett’s posthoc test for ** p < 0.01 compared to the Control group; ## p < 0.01 compared to the HG+HF group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36291547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ASC1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-89656 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASC1

Alternate Names

activating signal cointegrator 1, ASC-1, HsT17391, thyroid hormone receptor interactor 4, thyroid receptor interacting protein 4, Thyroid receptor-interacting protein 4, TR-interacting protein 4, TRIP-4

Gene Symbol

TRIP4

Additional ASC1 Products

Product Documents for ASC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ASC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ASC1 Antibody - BSA Free

Customer Reviews for ASC1 Antibody - BSA Free (1)

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  • ASC1 Antibody
    Name: Margherita verardo
    Application: Western Blot
    Sample Tested: Skeletal muscle
    Species: Human
    Verified Customer | Posted 07/13/2020
    We used a ProSieve QuadColor Protein Markers
    ASC1 Antibody - BSA Free NBP1-89656

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