ASPM Antibody
Novus Biologicals | Catalog # NB100-2278
![Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]](https://resources.rndsystems.com/images/products/ASPM-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2278-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Porcine, Bovine, Ovine
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 3425 and the C-terminus (residue 3477) of human ASP (Abnormal Spindle)-like, Microcephaly Associated using the numbering given in entry NP_060606.2 (GeneID 259266).
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 22350525).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ASPM Antibody
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]
Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.![Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]](https://resources.rndsystems.com/images/products/ASPM-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2278-img0002.jpg "Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]")
Immunocytochemistry/ Immunofluorescence: ASPM Antibody [NB100-2278]
Immunocytochemistry/Immunofluorescence: ASPM Antibody [NB100-2278] - Detection of Human ASPM in NBF-fixed asynchronous HeLa cells with anti-ASPM used at a dilution of 1:250. Detection: Goat anti-rabbit IgG (h&l) FITC used at a dilution of 1:100.Applications for ASPM Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Application Notes
For ICC/IF: Permeabilization with Triton-X 100 is recommended. Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.Likely to work with frozen sections. IHC was reported in scientific literature (PMID: 22350525). Use in IHC-P reported in scientific literature (PMID: 30092011).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.25 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ASPM
Alternate Names
ASP, asp (abnormal spindle) homolog, microcephaly associated (Drosophila), Asp homolog, Calmbp1, FLJ43117, MCPH5DKFZp686N06184, microcephaly associated (Drosophila), microcephaly, primary autosomal recessive 5
Entrez Gene IDs
259266 (Human)
Gene Symbol
ASPM
UniProt
Additional ASPM Products
Product Documents for ASPM Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ASPM Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for ASPM Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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