Bassoon Antibody (L124/59) [FL650] - Chimeric

Novus Biologicals | Catalog # NBP3-52406

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

FL650

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # L124/59
View all Bassoon Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (Uniprot#: O88778) produced recombinantly in E. Coli

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Description

FL650 Excitation: 654 nm, Emission: 672nm.
Each order is a unique lot. For specialized requirements, please inquire for available options.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.

Applications for Bassoon Antibody (L124/59) [FL650] - Chimeric

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Gene Symbol

BSN

Additional Bassoon Products

Product Documents for Bassoon Antibody (L124/59) [FL650] - Chimeric

Certificate of Analysis

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Product Specific Notices for Bassoon Antibody (L124/59) [FL650] - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Bassoon Antibody (L124/59) [FL650] - Chimeric

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  • Q: I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?

    A:

    After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

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