Bax Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56095

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bax Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 43-61 (PELALDPVPQDASTKKLSE) of human Bax alpha was used as immunogen, GenBank no. NP_620116.1. The immunogen sequence is 100% conserved in human Bax isoforms alpha (192 amino acids), beta (218 amino acids), epsilon (164 amino acids), sigma (179 amino acids), and psi (173 amino acids).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bax Antibody - BSA Free

Western Blot: Bax Antibody [NB100-56095]

Western Blot: Bax Antibody [NB100-56095]

Western Blot: Bax Antibody [NB100-56095] - Total protein from human THP1, A431, A549 and U2OS cells was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:2000 dilution of anti-BAX in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence
Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095]

Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095]

Immunohistochemistry-Paraffin: Bax Antibody [NB100-56095] - Formalin-fixed, paraffin-embedded sections of human colon cancer fom a tissue microarray stained for BAX using NB100-56095 at 1:2000. A. High Bax expression. B. Low Bax expression. Hematoxylin-eosin counterstain
Western Blot: Bax Antibody [NB100-56095]

Western Blot: Bax Antibody [NB100-56095]

Western Blot: Bax Antibody [NB100-56095] - Analysis of BAX in colon tumor cell lines using this antibody. Bax is detected in the SW620 and DLD-1 cell lines. However, Bax was not detected in the cell lines (LoVo, DU145, and LS180) known to have frameshift mutations in Bax (Rampino et al. 1997). An antibody to F1ATPase was used as a protein loading control.
Western Blot: Bax Antibody [NB100-56095]

Western Blot: Bax Antibody [NB100-56095]

Western Blot: Bax Antibody [NB100-56095] - Analysis of Bax in Daoy whole cell lysate using anti-Bax antibody. Image from verified customer review.
Bax Antibody - BSA Free

Western Blot: Bax Antibody - BSA Free [NB100-56095] -

Mitochondria dysregulation occurs due to PV administration. The 12Z cells were stimulated with PV for 18 h and then stained with TMRM or MitoSox dye. The mitochondrial membrane potential and mitochondrial reactive oxygen species were assessed using flow cytometry (A,B). Mitochondrial-dependent apoptosis signaling molecules, Bax and Bcl-2 were detected 24 h after PV treatment using western blotting (C). The specific molecular weight is represented on the left. Data are expressed as mean +/- SEM. Statistical analysis was conducted using a Dunnet one-way ANOVA (*; p < 0.05, **; p < 0.01, ***; p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35444541), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Bax Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Western Blot

1:1000-1:2000
Application Notes
Users should optimize according to model and immunodetection system used (secondary reagents)

Reviewed Applications

Read 1 review rated 5 using NB100-56095 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bax

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56095 recognizes Bax. It reacts with an epitope (PELALDPVPQDASTKKLSE)which is 100% conserved in human Bax isoforms alpha (192 amino acids), beta (218 amino acids), epsilon (164 amino acids), sigma (179 amino acids), and psi (173 amino acids).

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Entrez Gene IDs

581 (Human)

Gene Symbol

BAX

Additional Bax Products

Product Documents for Bax Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bax Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Bax Antibody - BSA Free

Customer Reviews for Bax Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Daoy whole cell lysate (25ug)
    Species: Human
    Verified Customer | Posted 07/14/2016
    Analysis of Bax in Daoy cell lysate (25ug)
    Bax Antibody - BSA Free NB100-56095

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