BCKDHA Antibody (HL2405) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25380

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2405 expressed in HEK293

Format

Azide and BSA Free
View all BCKDHA Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human BCKDHA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCKDHA Antibody (HL2405) - Azide and BSA Free

BCKDHA Antibody (HL2405) - Azide and BSA Free

Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -

Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
BCKDHA Antibody (HL2405) - Azide and BSA Free

Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -

Western Blot: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
BCKDHA Antibody (HL2405) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -

Immunocytochemistry/Immunofluorescence: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCKDHA stained by BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.
BCKDHA Antibody (HL2405) - Azide and BSA Free

Immunohistochemistry-Paraffin: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] -

Immunohistochemistry-Paraffin: BCKDHA Antibody (HL2405) - Azide and BSA Free [NBP3-25380] - BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. BCKDHA stained by BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BCKDHA Antibody (HL2405) - Azide and BSA Free

Immunohistochemistry: BCKDHA Antibody (HL2405) [NBP3-25380] -

BCKDHA antibody [HL2405] detects BCKDHA protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: BCKDHA stained by BCKDHA antibody [HL2405] (NBP3-25380) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

Applications for BCKDHA Antibody (HL2405) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCKDHA

BCKDHA - branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease)

Alternate Names

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDE1A, BCKDH E1-alpha, branched chain keto acid dehydrogenase E1, alpha polypeptide, branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urinedisease), Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC 1.2.4.4,2-oxoisovalerate dehydrogenase (lipoamide), FLJ45695, MSU, MSUD1, OVD1A

Gene Symbol

BCKDHA

Additional BCKDHA Products

Product Documents for BCKDHA Antibody (HL2405) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BCKDHA Antibody (HL2405) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BCKDHA Antibody (HL2405) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review BCKDHA Antibody (HL2405) - Azide and BSA Free and earn rewards!

Have you used BCKDHA Antibody (HL2405) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies