beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44346
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL9500
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of Q9P2W7, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
![beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]](https://resources.rndsystems.com/images/products/nbp3-44346_mouse-beta-1-3-glucuronyltransferase-1-mab-cl9500-28220251235197.jpg "Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]")
Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]
Staining of human duodenum shows moderate cytoplasmic positivity in lymphoid cells.![beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]](https://resources.rndsystems.com/images/products/nbp3-44346_mouse-beta-1-3-glucuronyltransferase-1-mab-cl9500-28220251344258.jpg "Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]")
Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]
Staining of human renal cancer shows moderate cytoplasmic positivity in lymphoid cells.![beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]](https://resources.rndsystems.com/images/products/nbp3-44346_mouse-beta-1-3-glucuronyltransferase-1-mab-cl9500-28220251331447.jpg "Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]")
Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]](https://resources.rndsystems.com/images/products/nbp3-44346_mouse-beta-1-3-glucuronyltransferase-1-mab-cl9500-282202513112866.jpg "Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]")
Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]
Staining of human prostate shows moderate to strong staining of secretion.![beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]](https://resources.rndsystems.com/images/products/nbp3-44346_mouse-beta-1-3-glucuronyltransferase-1-mab-cl9500-282202513381549.jpg "Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]")
Immunohistochemistry-Paraffin: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]
Staining of human tonsil shows strong cytoplasmic positivity in a small subset of lymphoid cells.![beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (CL9500) - Azide and BSA Free Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]](https://resources.rndsystems.com/images/products/nbp3-44346_mouse-beta-1-3-glucuronyltransferase-1-mab-cl9500-282202513245464.jpg "Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]")
Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP3-44346]
Analysis in human cerebral cortex tissue.Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: beta-1,3-Glucuronyltransferase 1/B3GAT1
Long Name
UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase
Alternate Names
CD57, GLCATP, GLCUATP, HNK1, LEU7
Gene Symbol
B3GAT1
Additional beta-1,3-Glucuronyltransferase 1/B3GAT1 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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