Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9)

Novus Biologicals | Catalog # NBP2-45527

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, SDS-Page

Cited:

Western Blot, SDS-Page

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3A9
View all Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human B3GNT2(NP_006568) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

45.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9)

Western Blot: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Western Blot: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Western Blot: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2.
Immunocytochemistry/ Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunocytochemistry/ Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunocytochemistry/Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527] - Analysis of HeLa cells.
Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunocytochemistry/ Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunocytochemistry/ Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunocytochemistry/Immunofluorescence: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2.
Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527]

Immunohistochemistry: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9) [NBP2-45527] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

SDS-Page

Reported in scientific publication (PMID: 32663509)

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2

B3GNT2 encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. [provided by RefSeq]

Long Name

UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1

Alternate Names

B3Galt6, Beta3gnt1

Gene Symbol

B3GNT2

Additional Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Products

Product Documents for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Antibody (OTI3A9)

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Protocols

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