beta COP Antibody

Name: beta COP Antibody
Brand: Novus Biologicals
SKU: NBP2-22544
Price: 169 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-22544

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Citations (2)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for beta COP Antibody

Western Blot: beta COP Antibody [NBP2-22544]

Western Blot: beta COP Antibody [NBP2-22544] - Analysis of beta-COP from rat brain homogenate.

Immunocytochemistry/ Immunofluorescence: beta COP Antibody [NBP2-22544]

Immunocytochemistry/Immunofluorescence: beta COP Antibody [NBP2-22544] - Immunolocalization of beta-COP in NIH-3T3 cells.

Applications for beta COP Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:10-1:500

Western Blot

1:1000

Application Notes

WB: Detects an approx. 110kDa protein which represents beta-COP from rat brain homogenate. Indirect IF: Staining of beta-COP in NIH-3T3 cells with NB120-2899 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

4.4 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta COP

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Alternate Names

beta coat protein, Beta-coat protein, Beta-COP, coatomer protein complex, subunit beta, coatomer protein complex, subunit beta 1, coatomer subunit beta, COPBFLJ57957, DKFZp761K102, FLJ10341, FLJ46444

Entrez Gene IDs

1315 (Human)

Gene Symbol

COPB1

UniProt

P53618 (Human), 70349 (Mouse), 114023 (Rat)

Additional beta COP Products

Product Documents for beta COP Antibody

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Certificate of Analysis

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Product Specific Notices for beta COP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta COP Antibody

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