Beta Dystroglycan Antibody (P38G1) - BSA Free

Novus Biologicals | Catalog # NBP3-43569

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # P38G1

Format

BSA Free
View all Beta Dystroglycan Primary Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing BALB/c mice with a mixture of transfected HEK 293 cells expressing human and mouse beta-DAG. (Uniprot# Q14118; Q62165)

Specificity

This antibody recognizes human and mouse beta dystroglycan (beta-DAG). Beta-dystroglycan is a transmembrane protein that plays an important role in connecting the extracellular matrix to the cytoskeleton. It plays an important role in linking dystrophin to the laminin-binding alpha-dystroglycan. It acts as a cell adhesion receptor in both muscle and non-muscle tissues. It also functions in cell adhesion-mediated signaling and implicated in cell polarity.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Beta Dystroglycan Antibody (P38G1) - BSA Free

Beta Dystroglycan Antibody (P38G1) - BSA Free Beta Dystroglycan Antibody (P38G1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] -

Immunocytochemistry/ Immunofluorescence: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on coverslips stained with the chimeric verison of this antibody, NBP3-43570 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell membrane/junction and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43570, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
Beta Dystroglycan Antibody (P38G1) - BSA Free Beta Dystroglycan Antibody (P38G1) - BSA Free

Flow Cytometry: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] -

Flow Cytometry: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] - MCF7 cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the chimeric version of this antibody, NBP3-43570 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Beta Dystroglycan Antibody (P38G1) - BSA Free Beta Dystroglycan Antibody (P38G1) - BSA Free

Western Blot: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] -

Western Blot: Beta Dystroglycan Antibody (P38G1) - BSA Free [NBP3-43569] - Human skeletal muscle tissue lysate (35µg protein in RIPA buffer) was resolved on an SDS-PAGE gel, and blots were probed with the chimeric verison of this antibody, NBP3-43570 at 0.1µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.

Applications for Beta Dystroglycan Antibody (P38G1) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Beta Dystroglycan

The dystroglycan complex is a membrane-spanning complex composed of two subunits, alpha- and beta-dystroglycan. Alpha-dystroglycan is a cell surface peripheral membrane protein which binds to the extracellular matrix (ECM), whereas beta-dystroglycan is an integral membrane protein which anchors alpha-dystroglycan to the cell membrane. The dystroglycan complex provides a tight link between the ECM and cell membrane. Dysfunction of the dystroglycan complex has commonly been implicated in the molecular pathogenesis of severe forms of hereditary neuromuscular diseases, including Duchenne muscular dystrophy, Fukuyama-type congenital muscular dystrophy and sarcoglycanopathy (1). Human dystroglycan is expressed in a variety of fetal and adult tissues. Data suggest that muscle and non-muscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Therefore variable glycosylation of the conserved protein core might modulate laminin binding (2). beta-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli (3).

Alternate Names

A3a, AGRNR, DAG156DAG, dystroglycan, dystroglycan 1 (dystrophin-associated glycoprotein 1), Dystrophin-associated glycoprotein 1

Gene Symbol

DAG1

Additional Beta Dystroglycan Products

Product Documents for Beta Dystroglycan Antibody (P38G1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Beta Dystroglycan Antibody (P38G1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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