beta Tubulin Antibody (TU-06) - BSA Free
Novus Biologicals | Catalog # NB120-7792
![Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792]](https://resources.rndsystems.com/images/products/beta-Tubulin-Antibody-TU-06-Western-Blot-NB120-7792-img0002.jpg "Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, A. thaliana, Chicken, Eukaryote, Fish, Plant
Cited:
Rat, Plant
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # TU-06
Format
BSA Free
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Product Specifications
Immunogen
Beta-subunits of porcine brain tubulin.
Reactivity Notes
Expected species cross-reactivity based on sequence homology: Carp, Bovine, Hamster, S.Cerevisiae, S.Pombe, and Protozoa. Plant reactivity reported in scientific literature (PMID: 26594217).
Localization
Cytoplasmic
Marker
Microtubule Marker
Specificity
The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for beta Tubulin Antibody (TU-06) - BSA Free
Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792]
Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792] - Analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06; dilution 0,5 ug/ml, 1 ug/ml, 2 ug/ml, 4 ug/ml, 5 ug/ml).![Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792]](https://resources.rndsystems.com/images/products/beta-Tubulin-Antibody-TU-06-Immunocytochemistry-Immunofluorescence-NB120-7792-img0003.jpg "Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792]")
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792]
Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy(R)5). Nucleus is stained with DAPI (blue).Applications for beta Tubulin Antibody (TU-06) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunocytochemistry: fixed and permeabilized cells; positive control: 3T3 mouse embryonal fibroblast cell line. Immunohistochemistry (paraffin sections) positive tissue: heart.
Reviewed Applications
Read 1 review rated 4 using NB120-7792 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
Tris buffered saline (TBS), pH 8.0
Format
BSA Free
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: beta Tubulin
Long Name
Tubulin beta chain
Alternate Names
OK/SW-cl.56, TUBB, TUBB5
Gene Symbol
TUBB
UniProt
Additional beta Tubulin Products
Product Documents for beta Tubulin Antibody (TU-06) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for beta Tubulin Antibody (TU-06) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for beta Tubulin Antibody (TU-06) - BSA Free
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Application: Western BlotSample Tested: rat liver cytosolSpecies: RatVerified Customer | Posted 01/22/2014
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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