![Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389]](https://resources.rndsystems.com/images/products/BOP1-Antibody-Immunohistochemistry-Paraffin-NBP1-21389-img0004.jpg "Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human block of proliferation 1 using the numbering given in entry NP_056016.1 (GeneID 23246).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BOP1 Antibody
Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389]
Immunohistochemistry-Paraffin: BOP1 Antibody [NBP1-21389] - FFPE section of human colon carcinoma (left) and mouse teratoma. Antibody:Affinity purified rabbit anti-BOP1 used at a dilution of 1:200 (1ug/ml). Detection: DAB![Immunoprecipitation: BOP1 Antibody [NBP1-21389]](https://resources.rndsystems.com/images/products/BOP1-Antibody-Immunoprecipitation-NBP1-21389-img0002.jpg "Immunoprecipitation: BOP1 Antibody [NBP1-21389]")
Immunoprecipitation: BOP1 Antibody [NBP1-21389]
Immunoprecipitation: BOP1 Antibody [NBP1-21389] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-21388 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.Applications for BOP1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BOP1
Alternate Names
block of proliferation 1, KIAA0124Block of proliferation 1 protein, ribosome biogenesis protein BOP1
Entrez Gene IDs
23246 (Human)
Gene Symbol
BOP1
UniProt
Additional BOP1 Products
Product Documents for BOP1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BOP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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