Bovine IL-4 Antibody (701214)

R&D Systems | Catalog # MAB2469

R&D Systems

Key Product Details

Species Reactivity

Bovine

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 701214
View all IL-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant bovine IL-4
His25-Cys135
Accession # P30367.2

Specificity

Detects bovine IL-4 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant porcine, canine, rhesus, cotton rat, equine, or rabbit IL-4 is observed and no cross-reactivity with recombinant human, mouse, rat, or feline IL-4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Bovine IL-4 Antibody (701214)

Detection of Bovine IL-4 antibody by Western Blot.

Detection of Bovine IL‑4 by Western Blot.

Western blot shows Recombinant Bovine IL-4 (Catalog # 2469-BL) (10 ng/lane). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IL-4 antibody in Bovine PBMCs by Immunocytochemistry (ICC).

IL‑4 in Bovine PBMCs.

IL-4 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) stimulated with concanavalin A using Mouse Anti-Bovine IL-4 Monoclonal Antibody (Catalog # MAB2469) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Bovine IL-4 Antibody (701214)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed bovine peripheral blood mononuclear bells (PBMCs) stimulated with concanavalin A

Western Blot

2 µg/mL
Sample: Recombinant Bovine IL‑4 (Catalog # 2469-BL)

Reviewed Applications

Read 1 review rated 5 using MAB2469 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Bovine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Alternate splicing generates two additional isoforms with internal deletions (5). Mature bovine IL-4 shares 60%, 91%, 93%, 78%, 55%, 39%, and 41% aa sequence identity with equine, goat, ovine, porcine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8‑11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Heussler, V.T. et al. (1992) Gene 114:273.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Waldvogel, A.S. et al. (2004) Vet. Immunol. Immunopathol. 97:53.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 751514 (Feline); 574281 (Primate); 100302454 (Rabbit)

Gene Symbol

IL4

UniProt

P30367.2

Additional IL-4 Products

Product Documents for Bovine IL-4 Antibody (701214)

Certificate of Analysis

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Product Specific Notices for Bovine IL-4 Antibody (701214)

For research use only

Citations for Bovine IL-4 Antibody (701214)

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  • Bovine IL-4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Bovine
    Verified Customer | Posted 06/27/2022
    Bovine IL-4 Antibody (701214) MAB2469

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