![Immunocytochemistry/ Immunofluorescence: BRD3 Antibody [NBP2-14360]](https://resources.rndsystems.com/images/products/BRD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14360-img0006.jpg "Immunocytochemistry/ Immunofluorescence: BRD3 Antibody [NBP2-14360]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: MPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSV
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRD3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BRD3 Antibody [NBP2-14360]
Immunocytochemistry/Immunofluorescence: BRD3 Antibody [NBP2-14360] - Staining of human cell line MCF7 shows localization to nuclear bodies. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]](https://resources.rndsystems.com/images/products/BRD3-Antibody-Immunohistochemistry-Paraffin-NBP2-14360-img0011.jpg "Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]")
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.![Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]](https://resources.rndsystems.com/images/products/BRD3-Antibody-Immunohistochemistry-NBP2-14360-img0007.jpg "Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]")
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.![Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]](https://resources.rndsystems.com/images/products/BRD3-Antibody-Immunohistochemistry-Paraffin-NBP2-14360-img0008.jpg "Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]")
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]](https://resources.rndsystems.com/images/products/BRD3-Antibody-Immunohistochemistry-Paraffin-NBP2-14360-img0010.jpg "Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]")
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360]
Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-14360] - Staining of human testis shows moderate nuclear psotivity in cells in seminiferous ducts.![BRD3 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: BRD3 Antibody - BSA Free [NBP2-14360]](https://resources.rndsystems.com/images/products/nbp2-14360_rabbit-polyclonal-brd3-antibody-25620259114090.jpg "Chromatin Immunoprecipitation-exo-Seq: BRD3 Antibody - BSA Free [NBP2-14360]")
Chromatin Immunoprecipitation-exo-Seq: BRD3 Antibody - BSA Free [NBP2-14360]
ChIP-Exo-Seq composite graph for Anti-BRD3 (NBP2-14360) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for BRD3 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BRD3
Long Name
Bromodomain Containing 3
Alternate Names
ORFX, RING3L
Gene Symbol
BRD3
Additional BRD3 Products
Product Documents for BRD3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRD3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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