Bu-1a Antibody (21-1A4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-28319

Novus Biologicals

Key Product Details

Species Reactivity

Chicken

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 21-1A4

Format

Azide and BSA Free
View all Bu-1a Primary Antibodies »

Product Specifications

Immunogen

Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens

Reactivity Notes

Chicken

Specificity

Chicken Bu-1a/b alloantigen, Mr 70 kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Bu-1a Antibody (21-1A4) - Azide and BSA Free

Flow Cytometry: Bu-1a Antibody (21-1A4) - Azide and BSA Free [NBP1-28319]

Flow Cytometry: Bu-1a Antibody (21-1A4) - Azide and BSA Free [NBP1-28319]

Flow Cytometry: Bu-1a Antibody (21-1A4) [NBP1-28319] - Analysis using the FITC conjugate of NBP1-28319. Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1a and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using light scatter criteria and analyzed on a flow cytometer.
Flow Cytometry: Bu-1a Antibody (21-1A4) - Azide and BSA Free [NBP1-28319]

Flow Cytometry: Bu-1a Antibody (21-1A4) - Azide and BSA Free [NBP1-28319]

Flow Cytometry: Bu-1a Antibody (21-1A4) [NBP1-28319] - Analysis using the Biotin conjugate of NBP1-28319. Multiple staining of peripheral blood mononuclear cells.

Applications for Bu-1a Antibody (21-1A4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

<= 1 ug/10^6 cells

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Bu-1a

Chicken Bu-1a/b, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence. (1-2)

Alternate Names

B6.1

Additional Bu-1a Products

Product Documents for Bu-1a Antibody (21-1A4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bu-1a Antibody (21-1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Bu-1a Antibody (21-1A4) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Bu-1a Antibody (21-1A4) - Azide and BSA Free and earn rewards!

Have you used Bu-1a Antibody (21-1A4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies