Bu-1a Antibody (21-1A4) [FITC]

Novus Biologicals | Catalog # NBP1-28320

Novus Biologicals

Key Product Details

Species Reactivity

Chicken

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 21-1A4
View all Bu-1a Primary Antibodies »

Product Specifications

Immunogen

Bursa cells from one-day-old H.B15 (Bu-1a/b) strain chickens

Reactivity Notes

Chicken

Specificity

Chicken Bu-1a/b alloantigen, Mr 70 kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Bu-1a Antibody (21-1A4) [FITC]

Flow Cytometry: Bu-1a Antibody (21-1A4) [FITC] [NBP1-28320] - Chicken peripheral blood mononuclear cells were first incubated with purified mouse anti-chicken Bu-1a and then counter-stained with goat anti-mouse IgG-FITC. Small lymphocytes were gated using light scatter criteria and analyzed on a FACScan (Trademark) flow cytometer (BDB, San Jose, CA).

Applications for Bu-1a Antibody (21-1A4) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Bu-1a

Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 21-1A4 does not react with cells from CHA and H.B15 strains by immunofluorescence. (1-2)

Alternate Names

B6.1

Additional Bu-1a Products

Product Documents for Bu-1a Antibody (21-1A4) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bu-1a Antibody (21-1A4) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Bu-1a Antibody (21-1A4) [FITC]

There are currently no reviews for this product. Be the first to review Bu-1a Antibody (21-1A4) [FITC] and earn rewards!

Have you used Bu-1a Antibody (21-1A4) [FITC]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies