CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)

Novus Biologicals | Catalog # NBP2-53220

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # CA19.9/1390R
View all CA19-9 Primary Antibodies »

Product Specifications

Immunogen

Purified human CA19-9/Sialyl Lewis A protein

Localization

Cytoplasmic

Marker

GI Tumor Marker

Specificity

CA19-9, a carbohydrate epitope expressed on a high MW (400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA and azide at 1 mg/ml.(NBP2-54585)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) [NBP2-53220]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) [NBP2-53220]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) [NBP2-53220] - CA19-9/Sialyl Lewis A antibody staining in human ductal carcinoma. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) [NBP2-53220]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) [NBP2-53220]

Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) [NBP2-53220] - Human Gastric Carcinoma stained with CA19-9 Recombinant Rabbit Monoclonal Antibody (CA19.9/1390R).

Applications for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 5-10ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-53220 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CA19-9

Mucin glucoprotein is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas.

Long Name

Carbohydrate Antigen 19-9

Alternate Names

CA 19-9

Additional CA19-9 Products

Product Documents for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)

Customer Reviews for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) (1)

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  • CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Ductal carcinoma
    Species: Human
    Verified Customer | Posted 10/11/2021
    CA19-9/Sialyl Lewis A Antibody swtaining in ductal carcinoma.
    Tissue sections were deparaffinized. Antigen retrieval was done enzymatically. Incubated O/N in 4 C with the primary antibody (1:200 dilution), followed by secondary antibody incubation (1:300 dilution).
    CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) NBP2-53220

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Protocols

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