CAD Antibody (0O8D6)

Novus Biologicals | Catalog # NBP3-16650

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0O8D6 expressed in HEK293
View all CAD Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CAD (P27708). MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CAD Antibody (0O8D6)

Western Blot: CAD Antibody (0O8D6) [NBP3-16650]

Western Blot: CAD Antibody (0O8D6) [NBP3-16650]

Western Blot: CAD Antibody (0O8D6) [NBP3-16650] - Western blot analysis of extracts of 293T cells, using CAD antibody (NBP3-16650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650]

Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650]

Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650]

Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650]

Immunocytochemistry/Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Immunofluorescence analysis of NIH-3T3 cells using CAD Rabbit mAb (NBP3-16650) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
CAD Antibody (0O8D6)

Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] -

Immunocytochemistry/ Immunofluorescence: CAD Antibody (0O8D6) [NBP3-16650] - Confocal imaging of HeLa cells using CAD Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Applications for CAD Antibody (0O8D6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CAD

Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).

Alternate Names

CAD protein, CAD trifunctional protein, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, anddihydroorotase, EC 2.1.3.2, EC 3.5.2.3, EC 6.3.5, EC 6.3.5.5, multifunctional protein CAD

Gene Symbol

CAD

Additional CAD Products

Product Documents for CAD Antibody (0O8D6)

Certificate of Analysis

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Product Specific Notices for CAD Antibody (0O8D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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