Calponin 1 Antibody (MSVA-455R)

Novus Biologicals | Catalog # NBP3-57212

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-455R
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Product Specifications

Immunogen

Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen. Swissprot P51911

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels..

Negative Control
Negitve control:Colon: Calponin 1 staining should be absent in all epithelial cells.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Calponin 1 Antibody (MSVA-455R)

Calponin 1 Antibody (MSVA-455R)

Immunohistochemistry-Paraffin:Calponin 1 Antibody (MSVA-455R)NBP3-57212

Breast
Calponin 1 Antibody (MSVA-455R)

Immunohistochemistry-Paraffin:Calponin 1 Antibody (MSVA-455R)NBP3-57212

Epididymis
Calponin 1 Antibody (MSVA-455R)

Immunohistochemistry-Paraffin:Calponin 1 Antibody (MSVA-455R)NBP3-57212

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Calponin 1 Antibody (MSVA-455R) Calponin 1 Antibody (MSVA-455R) Calponin 1 Cancer Tissue Gallery

Calponin 1 Cancer Tissue Gallery

Calponin 1 Antibody (MSVA-455R) Calponin 1 Antibody (MSVA-455R) Calponin 1 Cancer Tissue Gallery

Calponin 1 Cancer Tissue Gallery

Calponin 1 Antibody (MSVA-455R) Calponin 1 Antibody (MSVA-455R) Calponin 1 Normal Tissue Gallery

Calponin 1 Normal Tissue Gallery

Calponin 1 Antibody (MSVA-455R) Calponin 1 Antibody (MSVA-455R) Calponin 1 Normal Tissue Gallery

Calponin 1 Normal Tissue Gallery

Calponin 1 Antibody (MSVA-455R) Calponin 1 Antibody (MSVA-455R) Calponin 1 Normal Tissue Gallery

Calponin 1 Normal Tissue Gallery

Calponin 1 Antibody (MSVA-455R) Calponin 1 Antibody (MSVA-455R) Calponin 1 Normal Tissue Gallery

Calponin 1 Normal Tissue Gallery

Applications for Calponin 1 Antibody (MSVA-455R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calponin 1

Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction (1). Calponin binds filamentous actin (F-actin) through two distinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2) Immunoreactivity for calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adenocarcinomas (3).

Alternate Names

Calponin H1, CNN1, Sm-Calp, SMCC

Gene Symbol

CNN1

Additional Calponin 1 Products

Product Documents for Calponin 1 Antibody (MSVA-455R)

Certificate of Analysis

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Product Specific Notices for Calponin 1 Antibody (MSVA-455R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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