Canine CD34 APC‑conjugated Antibody

Name: Canine CD34 APC‑conjugated Antibody
Brand: R&D Systems
SKU: FAB3346A

R&D Systems | Catalog # FAB3346A

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Product Documents

Discontinued Product

FAB3346A has been discontinued. View all CD34 products.

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Canine

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1H6

View all CD34 Primary Antibodies »

Product Specifications

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34

Specificity

Detects canine CD34 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Canine CD34 APC‑conjugated Antibody

Detection of CD34 in CLL‑1390 Canine Cell Line by Flow Cytometry.

CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 APC-conjugated Monoclonal Antibody (Catalog # FAB3346A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Canine CD34 APC‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: CLL‑1390 canine leukocytic round cell neoplasia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD34

CD34 is a 100 kDa highly glycosylated member of the CD34 family of molecules. In dog, it is a 366 amino acid (aa) type I transmembrane protein that contains a 271 aa extracellular domain (ECD). Although originally identified on the surface of hematopoietic stem cells and vascular endothelial progenitors, it is now known to be expressed by highly divergent cell types such as telocytes/ICLC (cardiac support cells), cytotoxic NK lineage restricted progenitors, muscle satellite cells, corneal keratocytes (fibroblasts) and dendritic cell progenitors (with the exception of pre-conventional DCs). Little is known about CD34 ligands, save for L-Selectin. Canine CD34 ECD shares 60% aa sequence identity with human CD34 ECD.