Canine IFN‑ gamma Antibody

Name: Canine IFN‑ gamma Antibody
Brand: R&D Systems
SKU: AF781
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF781

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Citations (5)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Canine

Cited:

Mouse, Canine, Feline

Applications

Validated:

Western Blot, Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Neutralization, ELISpot Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all IFN-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant canine IFN-gamma
Gln24-Lys166
Accession # P42161

Specificity

Detects canine IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant porcine IFN‑ gamma is observed and less than 5% cross-reactivity with recombinant human IFN-gamma, recombinant rhesus macaque IFN-gamma, recombinant mouse IFN‑ gamma, and recombinant rat IFN‑ gamma is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Canine IFN‑ gamma Antibody

IFN‑ gamma Inhibition of VSV-induced Cytopathy and Neutralization by Canine IFN‑ gamma Antibody.

Recombinant Canine IFN-gamma (Catalog # 781-CG) reduces the Vesticular Stomatitis Virus (VSV)-induced cytopathy in the A-72 canine fibroma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of VSV activity elicited by Recombinant Canine IFN-gamma (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781). The ND₅₀ is typically 0.2-1 µg/mL.

IFN‑ gamma in Canine PBMCs.

IFN‑ gamma was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) untreated (lower panel) or treated with PMA and calcium ionomycin (upper panel) using Goat Anti-Canine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF781) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Canine IFN‑ gamma Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin

Western Blot

0.1 µg/mL
Sample: Recombinant Canine IFN-gamma (Catalog # 781-CG)

Neutralization

Measured by its ability to neutralize IFN‑ gamma inhibition of VSV-induced cytopathy in the A‑72 canine fibroma cell line. The Neutralization Dose (ND₅₀) is typically <8 µg/mL in the presence of 1.5 ng/mL Recombinant Canine IFN‑ gamma.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 79‑88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62‑73% with human, porcine, and rhesus IFN-gamma, and 40‑47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).