CARS Antibody (S01-7D4)

Novus Biologicals | Catalog # NBP3-19844

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S01-7D4 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide of human CARS (Uniprot # P49589)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARS Antibody (S01-7D4)

Western Blot: CARS Antibody (S01-7D4) [NBP3-19844]

Western Blot: CARS Antibody (S01-7D4) [NBP3-19844]

Western Blot: CARS Antibody (S01-7D4) [NBP3-19844] - Western blot detection of CARS in Hela, A549, HL-60, U2OS, C6 cell lysates using NBP3-19844 (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 86kDa.
Immunohistochemistry-Paraffin: CARS Antibody (S01-7D4) [NBP3-19844]

Immunohistochemistry-Paraffin: CARS Antibody (S01-7D4) [NBP3-19844]

Immunohistochemistry-Paraffin: CARS Antibody (S01-7D4) [NBP3-19844] - Immunohistochemistry of CARS in paraffin-embedded Human breast cancer tissue using NBP3-19844 at dilution 1/50

Applications for CARS Antibody (S01-7D4)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/2000-1/10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CARS

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

CARS1, CysRS, cysteine translase, cysteine tRNA ligase 1, cytoplasmic, Cysteine--tRNA ligase, cysteinyl-tRNA synthetase, cysteinyl-tRNA synthetase, cytoplasmic, EC 6.1.1, EC 6.1.1.16, MGC:11246

Gene Symbol

CARS1

Additional CARS Products

Product Documents for CARS Antibody (S01-7D4)

Certificate of Analysis

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Product Specific Notices for CARS Antibody (S01-7D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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