Caspase-8 Antibody
Novus Biologicals | Catalog # NB600-576
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576]](https://resources.rndsystems.com/images/products/Caspase-8-Antibody-Immunohistochemistry-Paraffin-NB600-576-img0002.jpg "Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant full length human Caspase 8 protein.
Localization
Cytoplasmic
Specificity
This reacts with a 55 kDa protein. Caspase 8 has an N-terminal domain with sequence homology to the death effector domain (DED) of FADD. Caspase 8 binds to DED of FADD. Caspase 8 is activated by association with Fas/FADD death-inducing signaling complex to release two active subunits, p18 and p10 into the cytosol, where they activate other caspases amplifying the apoptotic signal.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Caspase-8 Antibody
Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576]
Immunohistochemistry-Paraffin: Caspase-8 Antibody [NB600-576] - Formalin fixed paraffin embedded human stomach stained with Caspase-8 antibody (NB600-576).![Immunohistochemistry: Caspase-8 Antibody [NB600-576]](https://resources.rndsystems.com/images/products/Caspase-8-Antibody-Immunohistochemistry-NB600-576-img0001.jpg "Immunohistochemistry: Caspase-8 Antibody [NB600-576]")
Immunohistochemistry: Caspase-8 Antibody [NB600-576]
Immunohistochemistry: Caspase-8 Antibody [NB600-576] - Formalin-fixed, paraffin-embedded human stomach stained with Caspase 8. Note cytoplasmic staining of epithelial cells.Applications for Caspase-8 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50-1:100
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT. Use in Western Blot was reported in scientific literature.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 0.2% BSA, Tween-20
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Caspase-8
Alternate Names
CASP8, Caspase8, Mch5
Gene Symbol
CASP8
UniProt
Additional Caspase-8 Products
Product Documents for Caspase-8 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Caspase-8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Caspase-8 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Caspase-8 Antibody
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Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
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Associated Pathways
