Cathepsin D Antibody (SU0360)

Novus Biologicals | Catalog # NBP2-67477

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SU0360 expressed in HEK293
View all Cathepsin D Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Cathepsin D aa 363-412 / 412. (SwissProt: P07339 Human; SwissProt: P18242 Mouse)

Localization

Extracellular space, Lysosome, Melanosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cathepsin D Antibody (SU0360)

Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477]

Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477]

Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477] - Western blot analysis of Cathepsin D on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat An
Immunocytochemistry/ Immunofluorescence: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunocytochemistry/ Immunofluorescence: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunocytochemistry/Immunofluorescence: Cathepsin D Antibody (SU0360) [NBP2-67477] - Staining Cathepsin D in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunocytochemistry/ Immunofluorescence: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunocytochemistry/Immunofluorescence: Cathepsin D Antibody (SU0360) [NBP2-67477] - Staining Cathepsin D in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477] - Analysis of paraffin-embedded human gastric cancer tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477] - Analysis of paraffin-embedded human liver tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477] - Analysis of paraffin-embedded human lung tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477]

Immunohistochemistry-Paraffin: Cathepsin D Antibody (SU0360) [NBP2-67477] - Analysis of paraffin-embedded human pancreas tissue using anti-Cathepsin D antibody. Counter stained with hematoxylin.
Cathepsin D Antibody (SU0360) Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477]

Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477]

Western blot analysis on different lysates with NBP2-67477 at 1/2,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: Mouse brain tissue lysate (20 µg/Lane) Predicted band size: 45/28 kDa Observed band size: 45/28 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67477 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Cathepsin D Antibody (SU0360) Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477]

Western Blot: Cathepsin D Antibody (SU0360) [NBP2-67477]

Western blot analysis on different lysates with NBP2-67477 at 1/1,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 45/28 kDa Observed band size: 45/28 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67477 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for Cathepsin D Antibody (SU0360)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin D

Cathepsin D has been reported to be involved in malignant tumour progression and with prognosis of various human cancers.

Alternate Names

CTSD

Gene Symbol

CTSD

Additional Cathepsin D Products

Product Documents for Cathepsin D Antibody (SU0360)

Certificate of Analysis

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Product Specific Notices for Cathepsin D Antibody (SU0360)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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