Cathepsin S Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15727

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cathepsin S Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cathepsin S. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (85%), Bovine (80%).

Localization

Lysosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Cathepsin S Antibody - BSA Free (NBP2-15727) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cathepsin S Antibody - BSA Free

Western Blot: Cathepsin S Antibody [NBP2-15727]

Western Blot: Cathepsin S Antibody [NBP2-15727]

Western Blot: Cathepsin S Antibody [NBP2-15727] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Cathepsin S Antibody [NBP2-15727]

Immunocytochemistry/ Immunofluorescence: Cathepsin S Antibody [NBP2-15727]

Immunocytochemistry/Immunofluorescence: Cathepsin S Antibody [NBP2-15727] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using Cathepsin S antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: Cathepsin S Antibody [NBP2-15727]

Immunohistochemistry-Paraffin: Cathepsin S Antibody [NBP2-15727]

Immunohistochemistry-Paraffin: Cathepsin S Antibody [NBP2-15727] - Cathepsin S antibody detects Cathepsin S protein at lysosome on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. Cathepsin S antibody dilution: 1:500.
Cathepsin S Antibody

Western Blot: Cathepsin S Antibody [NBP2-15727] -

Western Blot: Cathepsin S Antibody [NBP2-15727] - Untreated (-) and treated (+) A549 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cathepsin S antibody (NBP2-15727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cathepsin S Antibody

Western Blot: Cathepsin S Antibody [NBP2-15727] -

Western Blot: Cathepsin S Antibody [NBP2-15727] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cathepsin S antibody (NBP2-15727) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Cathepsin S Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cathepsin S

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq]

Alternate Names

CTSS

Gene Symbol

CTSS

Additional Cathepsin S Products

Product Documents for Cathepsin S Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cathepsin S Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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