CD24 Antibody (4F4E10) - BSA Free

Novus Biologicals | Catalog # NBP2-37352

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F4E10

Format

BSA Free
View all CD24 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD24 (AA: 15-80) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD24 Antibody (4F4E10) - BSA Free (NBP2-37352) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD24 Antibody (4F4E10) - BSA Free

Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis using CD24 mAb against HEK293 (1) and CD24 (AA: 15-80)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD24 Antibody (4F4E10) - BSA Free [NBP2-37352]

Flow Cytometry: CD24 Antibody (4F4E10) - BSA Free [NBP2-37352]

Flow Cytometry: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis of Hela cells using CD24 mouse mAb (green) and negative control (red).
Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10)BSA Free [NBP2-37352]

Western Blot: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis using CD24 mAb against human CD24 recombinant protein. (Expected MW is 32.1 kDa)
ELISA: CD24 Antibody (4F4E10) - BSA Free [NBP2-37352]

ELISA: CD24 Antibody (4F4E10) - BSA Free [NBP2-37352]

ELISA: CD24 Antibody (4F4E10) [NBP2-37352] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CD24 Antibody (4F4E10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD24

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Alternate Names

CD24, CD24A

Gene Symbol

CD24

Additional CD24 Products

Product Documents for CD24 Antibody (4F4E10) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD24 Antibody (4F4E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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