CD39/ENTPD1 Antibody (JA90-36)

Novus Biologicals | Catalog # NBP2-67230

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JA90-36 expressed in HEK293
View all CD39/ENTPD1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CD39/ENTPD1 aa 371-420 / 510. (SwissProt: P49961 Human; SwissProt: P55772 Mouse; SwissProt: P97687 Rat)

Localization

Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD39/ENTPD1 Antibody (JA90-36)

Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Western blot analysis of CD39/ENTPD1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse heart tissue lysate Lane 2: mouse spleen tissue lysate
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD39/ENTPDI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human placenta tissue using anti-CD39 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human spleen tissue using anti-CD39 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded human tonsil tissue using anti-CD39 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Immunohistochemistry-Paraffin: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230] - Analysis of paraffin-embedded mouse uterus tissue using anti-CD39 antibody. Counter stained with hematoxylin.
CD39/ENTPD1 Antibody (JA90-36) Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Western Blot: CD39/ENTPD1 Antibody (JA90-36) [NBP2-67230]

Western blot analysis on different lysates with NBP2-67230 at 1/5,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse placenta tissue lysate (20 µg/Lane) Predicted band size: 58 kDa Observed band size: 70 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67230 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for CD39/ENTPD1 Antibody (JA90-36)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD39/ENTPD1

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 1

Alternate Names

ADPase, ATP-diphosphatase, CD39, ENTPD1, NTPD1

Gene Symbol

ENTPD1

Additional CD39/ENTPD1 Products

Product Documents for CD39/ENTPD1 Antibody (JA90-36)

Certificate of Analysis

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Product Specific Notices for CD39/ENTPD1 Antibody (JA90-36)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD39/ENTPD1 Antibody (JA90-36)

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Protocols

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