CD7 Antibody (MSVA-007R)

Novus Biologicals | Catalog # NBP3-57214

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-007R
View all CD7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human CD7 protein Swissprot P09564

Localization

Membranous

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Tonsil: A moderate to strong, predominantly membranous staining of T-cells in the interfollicular zones and the germinal centres should be seen. Colon: A moderate to strong staining of the intra-epithelial T-cells in the colon mucosa should be seen..

Negative Control
Negitve control:Tonsil/Colon: All epithelial structures should stain negative.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD7 Antibody (MSVA-007R)

CD7 Antibody (MSVA-007R)

Immunohistochemistry-Paraffin:CD7 Antibody (MSVA-007R)NBP3-57214

Colon Strong CD7 staining of a large fraction of tumor infiltrating lymphocytes in a colorectal adenocarcinoma. Tumor glands are completely CD7 negative.
CD7 Antibody (MSVA-007R)

Immunohistochemistry-Paraffin:CD7 Antibody (MSVA-007R)NBP3-57214

Strong membranous CD7 expression of an adenocarcinoma of the lung
CD7 Antibody (MSVA-007R)

Immunohistochemistry-Paraffin:CD7 Antibody (MSVA-007R)NBP3-57214

Tonsil Strong CD7 positivity of T lymphocytes mainly in the interfollicular area.

Applications for CD7 Antibody (MSVA-007R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (MSVA-007R)

Certificate of Analysis

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Product Specific Notices for CD7 Antibody (MSVA-007R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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