CD7 Antibody (rCD7/6972)

Novus Biologicals | Catalog # NBP3-20452

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rCD7/6972
View all CD7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa140-240) of human CD7 protein (exact sequence is proprietary)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Positive Controls: HePG-2 or Jurkat cells. Human lymph node or tonsil.

Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for CD7 Antibody (rCD7/6972)

CD7 Antibody (rCD7/6972) Multiplex Immunofluorescence: CD7 Antibody (rCD7/6972) [NBP3-20452]

CD7 in Human T-Cell Acute LymphoblasticLymphoma via seqIF™ staining on COMET™

CD7 was detected in immersion fixed paraffin-embedded sections of human T-cell Acute Lymphoblastic Lymphoma using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
CD7 Antibody (rCD7/6972) Multiplex Immunofluorescence: CD7 Antibody (rCD7/6972) [NBP3-20452]

CD7 in Human Appendix via seqIF™ stainingon COMET™

CD7 was detected in immersion fixed paraffin-embedded sections of human appendix using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog # NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
CD7 Antibody (rCD7/6972) Multiplex Immunofluorescence: CD7 Antibody (rCD7/6972) [NBP3-20452]

CD7 in Human Spleen via seqIF™ staining on COMET™

CD7 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog #NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​
CD7 Antibody (rCD7/6972) Multiplex Immunofluorescence: CD7 Antibody (rCD7/6972) [NBP3-20452]

CD7 in Human Thymus via seqIF™ staining on COMET™

CD7 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog #NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​
CD7 Antibody (rCD7/6972) Multiplex Immunofluorescence: CD7 Antibody (rCD7/6972) [NBP3-20452]

CD7 in Human Tonsil via seqIF™ staining on COMET™

CD7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD7, Monoclonal Antibody (Catalog #NBP3-20452) at 0.2ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​
CD7 Antibody (rCD7/6972)

CD7 Antibody (rCD7/6972)

Formalin-fixed, paraffin-embedded human tonsil stained with CD7 antibody (rCD7/6972). Inset: PBS instead of primary antibody; secondary only negative control.
CD7 Antibody (rCD7/6972)

CD7 Antibody (rCD7/6972)

Formalin-fixed, paraffin-embedded human lymph node stained with CD7 antibody (rCD7/6972). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for CD7 Antibody (rCD7/6972)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (rCD7/6972)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD7 Antibody (rCD7/6972)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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