CEA Antibody (MSVA-465R)

Novus Biologicals | Catalog # NBP3-57209

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-465R
View all CEA Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CEA protein Swissprot P06731

Localization

Cytoplasmic and luminal surface

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Colon: An at least moderate cytoplasmic CEA staining should be seen in the vast majority of columnar epithelial cells..

Negative Control
Negitve control:Colon: CEA staining should not be seen in stromal cells or smooth muscle.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CEA Antibody (MSVA-465R)

CEA Antibody (MSVA-465R)

Immunohistochemistry-Paraffin:CEA Antibody (MSVA-465R)NBP3-57209

Moderate to strong CEA immunostaining in the epithelial cells of the colon. Inflammatory and smooth muscle cells remain negative.
CEA Antibody (MSVA-465R)

Immunohistochemistry-Paraffin:CEA Antibody (MSVA-465R)NBP3-57209

Strong CEA immunostaining of the superfical layers of the squamous epithelium of the tonsil surface.The basal epithelial cell layers and inflammatory cells are CEA negative.
CEA Antibody (MSVA-465R)

Immunohistochemistry-Paraffin:CEA Antibody (MSVA-465R)NBP3-57209

Colorectal adenocarcinoma showing strong CEA immunostaining in tumor cells.

Applications for CEA Antibody (MSVA-465R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CEA

Alternate Names

Carcino Embryonic Antigen, Carcinoembryonic antigen

Additional CEA Products

Product Documents for CEA Antibody (MSVA-465R)

Certificate of Analysis

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Product Specific Notices for CEA Antibody (MSVA-465R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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