Cleavage stimulation factor 2 Antibody

Name: Cleavage stimulation factor 2 Antibody
Brand: Novus Biologicals
SKU: NB100-57531
Price: 459 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-57531

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 527 and 577 of human cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa using the numbering given in entry NP_001316.1 (GeneID 1478).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cleavage stimulation factor 2 Antibody

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531]

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57531] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-CSTF64 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531]

Immunohistochemistry-Paraffin: Cleavage stimulation factor 2 Antibody [NB100-57531] - Human ovarian carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1000 (1ug/ml).

Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531]

Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57531] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-57531 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57530, which recognizes an upstream epitope.

Applications for Cleavage stimulation factor 2 Antibody

Application

Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 mcg/mg

Application Notes

Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cleavage stimulation factor 2

This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Alternate Names

betaCstF-64 variant 2, CF-1 64 kDa subunit, Cleavage stimulation factor 64 kDa subunit, cleavage stimulation factor subunit 2, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD, cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, CSTF 64 kDa subunit, CstF-64

Entrez Gene IDs

1478 (Human)

Gene Symbol

CSTF2

UniProt

NP_001316.1 (Human)

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Product Specific Notices for Cleavage stimulation factor 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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