Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25417

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2048 + HL1907 expressed in HEK293

Format

Azide and BSA Free
View all Collagen I/II/III Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Collagen I/II/III.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Western Blot: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -

Western Blot: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] -

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Western Blot: Collagen I/II/III Antibody (HL2048 + HL1907) [NBP3-25417] -

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen I/II/III

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Alternate Names

Alpha-1 type I collagen, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, OI4, pro-alpha-1 collagen type 1

Gene Symbol

COL1A1

Additional Collagen I/II/III Products

Product Documents for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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