Complement Component C1qA Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-13331
![Western Blot: Complement Component C1qA Antibody [NBP3-13331]](https://resources.rndsystems.com/images/products/Complement-Component-C1qA-Antibody-Western-Blot-NBP3-13331-img0005.jpg "Western Blot: Complement Component C1qA Antibody [NBP3-13331]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of mouse Complement Component C1qA. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Complement Component C1qA Antibody - BSA Free
Western Blot: Complement Component C1qA Antibody [NBP3-13331]
Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Rat plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody [NBP3-13331]](https://resources.rndsystems.com/images/products/Complement-Component-C1qA-Antibody-Immunohistochemistry-Paraffin-NBP3-13331-img0002.jpg "Immunohistochemistry-Paraffin: Complement Component C1qA Antibody [NBP3-13331]")
Immunohistochemistry-Paraffin: Complement Component C1qA Antibody [NBP3-13331]
Immunohistochemistry-Paraffin: Complement Component C1qA Antibody [NBP3-13331] - Rat thymus gland. Complement Component C1qA stained by Complement Component C1qA antibody (NBP3-13331) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.![Western Blot: Complement Component C1qA Antibody [NBP3-13331]](https://resources.rndsystems.com/images/products/Complement-Component-C1qA-Antibody-Western-Blot-NBP3-13331-img0001.jpg "Western Blot: Complement Component C1qA Antibody [NBP3-13331]")
Western Blot: Complement Component C1qA Antibody [NBP3-13331]
Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Mouse plasma (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Western Blot: Complement Component C1qA Antibody [NBP3-13331]](https://resources.rndsystems.com/images/products/Complement-Component-C1qA-Antibody-Western-Blot-NBP3-13331-img0004.jpg "Western Blot: Complement Component C1qA Antibody [NBP3-13331]")
Western Blot: Complement Component C1qA Antibody [NBP3-13331]
Western Blot: Complement Component C1qA Antibody [NBP3-13331] - Human plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Complement Component C1qA antibody (NBP3-13331) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Complement Component C1qA Antibody [NBP3-13331] -
C1qA antibody detects C1qA protein at cytoplasm by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C1qA stained by C1qA antibody (NBP3-13331) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Applications for Complement Component C1qA Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Complement Component C1qA
Long Name
Complement Component 1q Subcomponent, A Chain
Alternate Names
C1QA
Gene Symbol
C1QA
Additional Complement Component C1qA Products
Product Documents for Complement Component C1qA Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Complement Component C1qA Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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