Cyclooxygenase (COX), also known as prostaglandin G/H synthase, is a membrane bound enzyme partly responsible for the catalysis of prostanoid synthesis. COX is expressed as at least three different isoforms. COX-1 is constitutively expressed and thought to regulate a number of 'housekeeping' functions such as vascular hemostasis, renal blood flow, and glomerular function. COX-2 expression is tightly regulated and induced by inflammatory mediators such as growth factors, cytokines, and endotoxin. COX-3 appears to be much more strictly regulated spatially and is observed in greatest abundance in cerebral cortex.
COX-1 Antibody (6B6) - BSA Free
Novus Biologicals | Catalog # NBP3-26362
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6B6 expressed in HEK293
Format
BSA Free
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Product Specifications
Immunogen
A synthesized peptide derived from Human COX-1 [UniProt P23219]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for COX-1 Antibody (6B6) - BSA Free
Western Blot: COX-1 Antibody (6B6) [NBP3-26362] -
Western Blot: COX-1 Antibody (6B6) [NBP3-26362] - Positive Western Blot detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissueAll lanes: COX-1 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 69, 65, 62, 57, 72, 73 kDa
Observed band size: 72 kDa
Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] -
Flow Cytometry: COX-1 Antibody (6B6) [NBP3-26362] - Overlay histogram showing Hela cells stained with COX-1 Antibody (6B6) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for COX-1 Antibody (6B6) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:20-1:200
Western Blot
1:500-1:5000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: COX-1
Long Name
Cyclooxygenase 1
Alternate Names
COX1, PTGS1
Gene Symbol
PTGS1
Additional COX-1 Products
Product Documents for COX-1 Antibody (6B6) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for COX-1 Antibody (6B6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for COX-1 Antibody (6B6) - BSA Free
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Q: Please could you tell me if Novus Biologicals has an active COX1 Recombinant Protein?
A: Unfortunately we do not currently have any COX1 recombinant proteins that are expected to be active.
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