CTCF Antibody

Novus Biologicals | Catalog # NB500-177

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CTCF Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 650 and 700 of human CCCTC-binding factor using the numbering given in entry NP_006556.1 (GeneID 10664).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CTCF Antibody (NB500-177) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-CTCF Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CTCF Antibody

Western Blot: CTCF Antibody [NB500-177]

Western Blot: CTCF Antibody [NB500-177]

Western Blot: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177]

Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177]

Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.
Chromatin Immunoprecipitation: CTCF Antibody [NB500-177] - Analysis of Human CTCF by Chromatin Immunoprecipitation (ChIP). Primary human fibroblasts from lung (5 x 107 cells) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 20 micrograms of rabbit anti-CTCF antibody NB500-177. The resulting ChIP DNA was quantified using real-time PCR with primers against the IGF2-H19 ICR or a control region in the HoxA cluster.
Western Blot: CTCF Antibody [NB500-177]

Western Blot: CTCF Antibody [NB500-177]

Western Blot: CTCF Antibody [NB500-177] - Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CTCF antibody NB500-177 used for WB at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177]

Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177]

Immunohistochemistry-Paraffin: CTCF Antibody [NB500-177] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CTCF antibody (NB500-177) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunoprecipitation: CTCF Antibody [NB500-177]

Immunoprecipitation: CTCF Antibody [NB500-177]

Immunoprecipitation: CTCF Antibody [NB500-177] - Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CTCF antibody NB500-177 (lot NB500-177-6) used for IP at 6 ug per reaction. CTCF was also immunoprecipitated by a previous lot of this antibody (lot NB500-177-5). For blotting immunoprecipitated CTCF, NB500-177 was used at 0.02 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for CTCF Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2500-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. Chip, WB, IP reacivity reported in (PMID: 24741094).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CTCF

The vertebrate insulator protein, CCCTC binding factor (CTCF) is a highly conserved, ubiquitously expressed DNA-binding protein that has widespread capabilities in gene regulation. CTCF contains 11 zinc finger motifs and it is thought that CTCF uses different combinations of zinc fingers to recognise a variety of specific sequences on the DNA to control several important genes. CTCF plays dual roles in cell growth regulatory processes and epigenetic functions. CTCF is a negative regulator of cell proliferation and is located in a region of the chromosome that is frequently deleted in human cancers. The CTCF protein is altered in some breast and prostate tumours. Substitutions from one amino acid to another in the DNA-binding domain change the whole spectrum of the genes regulated by CTCF which can result in cancer development. CTCF was first identified as one of the nuclear factors binding to the regulatory regions of the c-myc gene. Further study of this protein revealed roles in transcriptional repression, insulator function, and imprinting genetic information. Among the multitude of normal CTCF functions, its involvement in partitioning the genome into active or inactive domains by means of a chromatin insulator function has received particular attention. As an insulator CTCF helps to define the boundaries between the neighbouring genes. Strikingly, all vertebrate chromatin insulators identified so far interact with CTCF.

Long Name

CCCTC-binding Factor

Alternate Names

11-Zinc Finger Protein2, CCCTC-binding Factor

Entrez Gene IDs

10664 (Human)

Gene Symbol

CTCF

UniProt

P49711 (Human)

Additional CTCF Products

Product Documents for CTCF Antibody

Certificate of Analysis

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Product Specific Notices for CTCF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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