Cytokeratin 13 Antibody (MSVA-613M) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-57298

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-613M

Format

Azide and BSA Free
View all Cytokeratin 13 Primary Antibodies »

Product Specifications

Immunogen

esophageal keratins of rabbit origin Swissprot P13646

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Tonsil: All squamous epithelial cells of the surface squamous epithelium (except the basal layer) and a fraction of squamous cells of the crypts should show strong KRT13 staining.

Negative Control
Negitve control:Tonsil: All lymphocytes and blood vessels must not show any KRT13 staining.

Applications for Cytokeratin 13 Antibody (MSVA-613M) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 13

Alternate Names

CK13, CK-13, cytokeratin 13, Cytokeratin-13, K13cytokeratin-13, keratin 13, keratin, type I cytoskeletal 13, keratin-13, MGC161462, MGC3781

Gene Symbol

KRT13

Additional Cytokeratin 13 Products

Product Documents for Cytokeratin 13 Antibody (MSVA-613M) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 13 Antibody (MSVA-613M) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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