Cytokeratin 15 Antibody (KRT15/2959)
Novus Biologicals | Catalog # NBP3-07620
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2c Kappa Clone # KRT15/2959
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Product Specifications
Immunogen
Recombinant full-length human Cytokeratin 15 protein (Uniprot: P19012)
Localization
Cytoplasmic
Marker
Esophageal Squamous Cell Carcinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2c Kappa
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08599)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytokeratin 15 Antibody (KRT15/2959)
![Western Blot: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]](https://resources.rndsystems.com/images/products/Cytokeratin-15-Antibody-KRT15-2959-Western-Blot-NBP3-07620-img0005.jpg "Western Blot: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]")
Western Blot: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]
Western Blot: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620] - Western Blot Analysis of Human Thymus tissue lysate using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959)![Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]](https://resources.rndsystems.com/images/products/Cytokeratin-15-Antibody-KRT15-2959-Immunocytochemistry-Immunofluorescence-NBP3-07620-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]")
Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]
Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620] - Confocal immunofluorescence image of MCF-7 cells using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959) followed by goat anti- Mouse CF488 (Cyan) and RedDot is used to label the nuclei Red.![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]](https://resources.rndsystems.com/images/products/Cytokeratin-15-Antibody-KRT15-2959-Immunohistochemistry-Paraffin-NBP3-07620-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]")
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]](https://resources.rndsystems.com/images/products/Cytokeratin-15-Antibody-KRT15-2959-Immunohistochemistry-Paraffin-NBP3-07620-img0003.jpg "Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]")
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959)![Protein Array: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]](https://resources.rndsystems.com/images/products/Cytokeratin-15-Antibody-KRT15-2959-Protein-Array-NBP3-07620-img0001.jpg "Protein Array: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]")
Protein Array: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620]
Protein Array: Cytokeratin 15 Antibody (KRT15/2959) [NBP3-07620] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959).Applications for Cytokeratin 15 Antibody (KRT15/2959)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/million cells
Immunohistochemistry-Frozen
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Frozen & formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 15
Long Name
keratin 15
Alternate Names
CK15, CK-15, cytokeratin 15, cytokeratin-15, K15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15
Gene Symbol
KRT15
UniProt
Additional Cytokeratin 15 Products
Product Documents for Cytokeratin 15 Antibody (KRT15/2959)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 15 Antibody (KRT15/2959)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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