Cytokeratin 15 Antibody (MSVA-615M)

Novus Biologicals | Catalog # NBP3-57231

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MSVA-615M
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Product Specifications

Immunogen

Recombinant full-length human KRT15 protein Swissprot P19012

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Tonsil: All cells of the surface squamous epithelium and most squamous cells of the crypts should show strong KRT15 staining..

Negative Control
Negitve control:Tonsil: All lymphocytes and blood vessels must not show any KRT15 staining.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cytokeratin 15 Antibody (MSVA-615M)

Cytokeratin 15 Antibody (MSVA-615M)

Immunohistochemistry-Paraffin:Cytokeratin 15 Antibody (MSVA-615M)NBP3-57231

Esophagus squamous epithelium A strong KRT15 immunostaining is always found in all cell layers of all non keratinizing and keratinizing squamous epithelium
Cytokeratin 15 Antibody (MSVA-615M)

Immunohistochemistry-Paraffin:Cytokeratin 15 Antibody (MSVA-615M)NBP3-57231

Tonsil surface Epithelium A strong KRT15 immunostaining is found in all cell layers of all non keratinizing and keratinizing squamous epithelium except keratin layer
Cytokeratin 15 Antibody (MSVA-615M)

Immunohistochemistry-Paraffin:Cytokeratin 15 Antibody (MSVA-615M)NBP3-57231

Tonsil In tonsil krypts a strong KRT15 immunostaining is found in all cell layers of squamous epithelium

Applications for Cytokeratin 15 Antibody (MSVA-615M)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 1:100
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 15

Long Name

keratin 15

Alternate Names

CK15, CK-15, cytokeratin 15, cytokeratin-15, K15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15

Gene Symbol

KRT15

Additional Cytokeratin 15 Products

Product Documents for Cytokeratin 15 Antibody (MSVA-615M)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cytokeratin 15 Antibody (MSVA-615M)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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