Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48114

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Ferret, Hamster, Sheep, Xenopus (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM268

Format

IHC-Prediluted
View all Cytokeratin 5/8 Primary Antibodies »

Product Specifications

Immunogen

Cytoskeletal preparation from HeLa cells

Reactivity Notes

Does not react with Chicken or Xenopus.

Localization

Cytoplasmic

Specificity

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114]

Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114]

Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114] - Human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (SPM268).

Applications for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS, pH 7.4 with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 5/8

Alternate Names

cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5

Gene Symbol

KRT5

Additional Cytokeratin 5/8 Products

Product Documents for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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