Cytokeratin 8 Antibody (OTI1B12)

Novus Biologicals | Catalog # NBP1-48281

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B12
View all Cytokeratin 8 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Marker

Epithelial Cell Marker

Specificity

This antibody is specific for Homo sapiens keratin 8 (KRT8).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 8 Antibody (OTI1B12)

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Cytokeratin 8 antibody staining of mouse colon epithelium. IHC-P image submitted by a verified customer review.
Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokeratin 8.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of A549 cells using anti-Cytokeratin 8 mouse monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Immunofluorescent staining of HeLa cells using anti-Cytokeratin 8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
Cytokeratin 8 Antibody (OTI1B12)

Cytokeratin 8 Antibody (OTI1B12)[NBP1-48281]

Cytokeratin 8 Antibody (OTI1B12)[NBP1-48281]Simple Western� analysis of endogenous protein NNMT from HELA lysates (0.5 mg/mL) using NNMT Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for Cytokeratin 8 Antibody (OTI1B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2500-5000

Reviewed Applications

Read 1 review rated 5 using NBP1-48281 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Alternate Names

CARD2, CK8, EndoA, KRT8

Entrez Gene IDs

3856 (Human)

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody (OTI1B12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 8 Antibody (OTI1B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 8 Antibody (OTI1B12)

Customer Reviews for Cytokeratin 8 Antibody (OTI1B12) (1)

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  • Cytokeratin 8 Antibody (OTI1B12)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Colon epithelium
    Species: Mouse
    Verified Customer | Posted 11/12/2021
    Cytokeratin 8 Antibody in mouse colon epithelium
    Primary antibody dilution: 1:200. Secondary antibody dilution: 1:300.
    Cytokeratin 8 Antibody (OTI1B12) NBP1-48281

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Protocols

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